Serum samples can be substituted by plasma samples for the diagnosis of paratuberculosis
- Format
- Article
- Status
- publishedVersion
- Description
Employing plasma samples rather than serum samples for serological paratuberculosis diagnosis is practical, especially when bovine TB is assessed in the same cattle herd with the gamma interferon bovine avian (IFN- BA) test. We demonstrate that antibody titers in serum and plasma samples, utilizing the PARACHECK® ELISA kit, are highly comparable (Cohen’s kappa test, k = 0.955). We conclude that serum can be replaced with plasma in this commercially available antibody detection assay resulting in working hour savings for sampling and blood sample work-up and cost reductions for materials and sample storage.
Employing plasma samples rather than serum samples for serological paratuberculosis diagnosis is practical, especially when bovine TB is assessed in the same cattle herd with the gamma interferon bovine avian (IFN- BA) test. We demonstrate that antibody titers in serum and plasma samples, utilizing the PARACHECK® ELISA kit, are highly comparable (Cohen’s kappa test, k = 0.955). We conclude that serum can be replaced with plasma in this commercially available antibody detection assay resulting in working hour savings for sampling and blood sample work-up and cost reductions for materials and sample storage.
- Publication Year
- 2013
- Language
- eng
- Topic
- Bovine tuberculosis
Paratuberculosis
Diagnosis
ELISA
PARACHECK®
Biomarker
- Repository
- RI INDICASAT
- Get full text
- https://doi.org/10.1016/j.prevetmed.2013.06.011
http://repositorio-indicasat.org.pa/handle/123456789/139
- Rights
- openAccess
- License