Plasmodium falciparum field isolates use complement receptor 1 (CR1) as a receptor for invasion of erythrocytes
- Authors
- Awandarea, Gordon A.; Spadafora, Carmenza; Mocha, J. Kathleen; Duttaa, Sheetij; Haynesa, J. David; Stoutea, José A.
- Format
- Article
- Status
- publishedVersion
- Description
AmajorityofPlasmodiumfalciparumstrainsinvadeerythrocytesthroughinteractionswithsialicacid(SA) on glycophorins. However, we recently reported that complement receptor 1 (CR1) is a SA-independent invasionreceptorofmanylaboratorystrainsofP.falciparum.TodeterminetheroleofCR1inerythrocyte invasion among P. falciparum field isolates, we tested eight isolates obtained from children in Kenya. All the parasites examined were capable of invading in a SA-independent manner, and invasion of neuraminidase-treatederythrocyteswasnearlycompletelyblockedbyanti-CR1andsolubleCR1(sCR1). Inaddition,anti-CR1andsCR1partiallyinhibitedinvasionofintacterythrocytesinamajorityofisolates tested. Sequencing of the hypervariable region of P. falciparum AMA-1 showed considerable diversity among all the isolates. These data demonstrate that CR1 mediates SA-independent erythrocyte invasion in P. falciparum field isolates.
AmajorityofPlasmodiumfalciparumstrainsinvadeerythrocytesthroughinteractionswithsialicacid(SA) on glycophorins. However, we recently reported that complement receptor 1 (CR1) is a SA-independent invasionreceptorofmanylaboratorystrainsofP.falciparum.TodeterminetheroleofCR1inerythrocyte invasion among P. falciparum field isolates, we tested eight isolates obtained from children in Kenya. All the parasites examined were capable of invading in a SA-independent manner, and invasion of neuraminidase-treatederythrocyteswasnearlycompletelyblockedbyanti-CR1andsolubleCR1(sCR1). Inaddition,anti-CR1andsCR1partiallyinhibitedinvasionofintacterythrocytesinamajorityofisolates tested. Sequencing of the hypervariable region of P. falciparum AMA-1 showed considerable diversity among all the isolates. These data demonstrate that CR1 mediates SA-independent erythrocyte invasion in P. falciparum field isolates.
- Publication Year
- 2011
- Language
- eng
- Topic
- Malaria
CR1
Red Cell
Invasion
Sialic acid
- Repository
- RI INDICASAT
- Get full text
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070236/
http://repositorio-indicasat.org.pa/handle/123456789/60
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/